K-Set ʻike i ka IMP kūʻē i ka Carbapenem (Lateral Flow Assay)
Hoʻolauna Huahana
ʻO ka Carbapenem-resistant IMP Detection K-Set (Lateral Flow Assay) he ʻōnaehana hoʻāʻo immunochromatographic i manaʻo ʻia no ka ʻike qualitative o IMP-type carbapenemase i loko o nā kolone bacteria.ʻO ka assay kahi hōʻike hoʻohana ʻana i ka lāʻau lapaʻau e hiki ke kōkua i ka ʻike ʻana i nā ʻano IMP-type carbapenem resistant strains.
Nā hiʻohiʻona
| inoa | K-Set ʻike i ka IMP kūʻē i ka Carbapenem (Lateral Flow Assay) |
| Kaʻina hana | Lateral Flow Assay |
| ʻAno laʻana | ʻO nā kolo bacteria |
| Hōʻike | 25 ho'āʻo/kit |
| Ka manawa ʻike | 10-15 min |
| Nā mea ʻike | ʻO Enterobacteriaceae (CRE) kūʻē i ka Carbapenem |
| ʻAno ʻike | IMP |
| Paʻa | Paʻa ka K-Set no 2 mau makahiki ma 2°C-30°C |
Pōmaikaʻi
- wikiwiki
Loaʻa ka hopena i loko o 15 min, 3 mau lā ma mua o nā hana ʻike kuʻuna - Maʻalahi
Hiki ke hoʻohana maʻalahi, hiki i nā limahana hana maʻamau ke hana me ka ʻole o ke aʻo ʻana - pololei
ʻO ka ʻike kiʻekiʻe a me ka kikoʻī
palena ʻike haʻahaʻa: 0.20 ng/mL
Hiki ke ʻike i ka hapa nui o nā subtypes maʻamau o IMP
- Ka hopena intuitive
ʻAʻohe pono no ka helu ʻana, ka hopena heluhelu ʻike - Waiwai
Hiki ke lawe ʻia a mālama ʻia ka huahana ma ka lumi wela, e hōʻemi ana i nā kumukūʻai
ʻO ke koʻikoʻi o ka hōʻike CRE
ʻO ka hui pū ʻana, ʻo Enterobacterales ka hui maʻamau o nā pathogens e hoʻoulu ai i nā maʻi e pili ana i ka mālama olakino.Hiki i kekahi mau Enterobacterales ke hana i kahi enzyme i kapa ʻia he carbapenemase e hana ʻole ai i nā lāʻau antibiotic e like me carbapenems, penicillins, a me nā cephalosporins.No kēia kumu, ua kapa ʻia ʻo CRE he "nightmare bacteria" no ka mea he kakaikahi nā lāʻau ʻokoʻa, inā he, i waiho ʻia e mālama i nā maʻi i hoʻokumu ʻia e kēia mau maʻi.
Hiki i nā maʻi bacteria mai ka ʻohana Enterobacterales, me nā ʻano Klebsiella a me Escherichia coli, ke hana i kahi carbapenemase.Hoʻopuka pinepine ʻia nā Carbapenemases mai nā genes i loaʻa ma nā mea hiki ke hoʻololi ʻia e hiki ke hoʻolaha maʻalahi i ke kū'ē ʻana mai ka germ a i ka germ a me ke kanaka i ke kanaka.Ma muli o ka hoʻohana ʻino ʻana i nā lāʻau antibiotic a me nā ala liʻiliʻi i hana ʻia e pale ai i ka laha ʻana, ua lilo ka pilikia CRE i mea weliweli i ke ola honua.
ʻO ka maʻamau, hiki ke mālama ʻia ka laha ʻana o CRE e:
- Nānā i nā maʻi CRE
- E hoʻokaʻawale i nā maʻi me CRE
- Wehe i nā lāʻau lapaʻau invasive i loko o ke kino
- E akahele i ke kau ʻana i nā lāʻau antibiotic (ʻoi aku ka carbapenems)
- Ke hoʻohana nei i nā ʻenehana sterile maʻemaʻe e hōʻemi i ka laha ʻana o ka maʻi
- E hahai pono i ka hana hoʻomaʻemaʻe lab
……
He waiwai nui ka ʻike CRE i ka mana hoʻolaha.Ma ka hoʻāʻo mua ʻana, hiki i nā mea mālama olakino ke hāʻawi i kahi lāʻau kūpono i nā poʻe maʻi i hiki ke loaʻa iā CRE, e hoʻokō pū i ka hoʻokele hoʻokipa.
IMP-type carbapenemase
ʻO ka Carbapenemase e pili ana i kahi ʻano β-lactamase hiki ke hoʻoheheʻe nui i ka imipenem a i ʻole meropenem, me ka A, B, D ʻekolu mau ʻano enzyme i hoʻokaʻawale ʻia e Ambler molecular structure.Ma waena o lākou, ʻo ka papa B he metallo-β-lactamases (MBLs), me nā carbapenemases e like me IMP, VIM a me NDM,.ʻO ka carbapenemase ʻano IMP, i ʻike ʻia ʻo imipenemase metallo-beta-lactamase e hana ana i ka CRE, he ʻano maʻamau loa ia o nā MBL i loaʻa a mai ka subclass 3A.Hiki iā ia ke hydrolyze kokoke i nā lāʻau antibiotic β-lactam.
Hana
- Hoʻohui i 5 kulu o ka hāpana lāʻau lapaʻau
- E hoʻopaʻa i nā ʻāpana bacteria me kahi loop inoculation disposable
- E hoʻokomo i ka loop i loko o ka paipu
- E hoʻohui i 50 μL i ka punawai S, e kali no 10-15 mau minuke
- E heluhelu i ka hopena
ʻIke Kauoha
| Hoʻohālike | wehewehe | Code huahana |
| CPI-01 | 25 ho'āʻo/kit | CPI-01 |
