ʻO ka hoʻihoʻi hou ʻana o ka maʻi HIV-1 Latent e ka Periodontopathic Bacterium

ʻO nā pūnaewele i hoʻopaʻa ʻia i ka maʻi i hoʻopaʻa ʻia i ka genome DNA proviral HIV-1 i hoʻopili mua ʻia i loko o ka heterochromatin, e ʻae ana i ka hoʻomau ʻana o nā proviruses transcriptionally silent.Hoʻopili ʻia ka hypoacetylation o nā protein histone e nā histone deacetylases (HDAC) i ka mālama ʻana i ka latency HIV-1 ma ke kāohi ʻana i ka transcription viral.Eia kekahi, ʻo nā maʻi periodontal, i hoʻokumu ʻia e ka polymicrobial subgingival bacteria me Porphyromonas gingivalis, ʻo ia kekahi o nā maʻi maʻamau o ke kanaka.Maanei mākou e hōʻike ai i nā hopena o P. gingivalis i ka hoʻopiʻi HIV-1.Hiki ke hoʻopili ʻia kēia haʻawina i ka supernatant moʻomeheu bacteria akā ʻaʻole i nā mea bacteria ʻē aʻe e like me fimbriae a i ʻole LPS.Ua ʻike mākou ua hoʻihoʻi ʻia kēia hana HIV-1-inducing ma ka haʻahaʻa molecular mass (<3 kDa) hapa o ka supernatant moʻomeheu.Ua hōʻike pū mākou i ka P. gingivalis e hoʻopuka i nā kiʻekiʻe kiʻekiʻe o ka butyric acid, e hana ana ma ke ʻano he mea paʻa ikaika o nā HDAC a hoʻoulu i ka histone acetylation.Ua hōʻike ʻia ʻo Chromatin immunoprecipitation assays ua hoʻokaʻawale ʻia ka corepressor complex i loaʻa HDAC1 a me AP-4 mai ka HIV-1 long terminal repeat promoter ma ka hoʻoulu ʻana me ka supernatant moʻomeheu bacteria me ka hui pū ʻana o ka histone acetylated a me RNA polymerase II.No laila ua ʻike mākou he hiki i ka P. gingivalis ke hoʻoulu i ka HIV-1 ma o ka hoʻololi ʻana i ka chromatin a ʻo ka butyric acid, kekahi o nā metabolites bacteria, ke kuleana o kēia hopena.Hōʻike kēia mau hopena e hiki ke hana i nā maʻi periodontal ma ke ʻano he kumu pilikia no ka hoʻōla hou ʻana o ka HIV-1 i nā poʻe maʻi a hiki ke kōkua i ka hoʻolaha pūnaewele o ka maʻi.