ʻIke pololei o ka Antibody Viral

Hoʻohana ʻia kēia mau ʻano hana i ka hoʻohana ʻana i ka antigen viral kūikawā e ʻike ai i nā antibodies i ka serum o nā mea maʻi, me ka ʻike ʻana i nā antibodies IgM a me ke ana ʻana o nā antibodies IgG.Nalo nā antibodies IgM i nā pule he nui, akā hoʻomau nā antibodies IgG no nā makahiki he nui.ʻO ka hoʻokumu ʻana i ka hōʻailona o kahi maʻi maʻi i hoʻokō ʻia ma ka serologically ma ka hōʻike ʻana i ka piʻi ʻana o ka titer antibody i ka maʻi a i ʻole ma ka hōʻike ʻana i nā antibodies antiviral o ka papa IgM.ʻO nā ʻano hana i hoʻohana ʻia me ka hoʻāʻo neutralization (Nt), ka hoʻāʻo complement fixation (CF), ka hoʻāʻo ʻana i ka hemagglutination inhibition (HI), a me ka hoʻāʻo immunofluorescence (IF), passive hemagglutination, a me immunodiffusion.

A. Nā ho'āʻo hoʻohālikelike

I ka wā o ka maʻi a i ʻole ka moʻomeheu cell, hiki ke pale ʻia ka maʻi virus e kāna antibody kikoʻī a nalowale ka infectivity, ua wehewehe ʻia kēia ʻano antibody he antibody neutralization.ʻO ka neutralization assays ka ʻike ʻana i ka antibody neutralization i ka serum o nā maʻi.

B. Hoʻohui i nā ho'āʻo hoʻoponopono

Hiki ke hoʻohana ʻia ka ho'āʻo hoʻoponopono hoʻohui e ʻimi i ke kū ʻana o kahi antibody kūikawā a i ʻole antigen i loko o ka serum o ka mea maʻi.Hoʻohana ka hoʻāʻo ʻana i nā sela koko ʻulaʻula hipa (SRBC), anti-SRBC antibody a hoʻohui pū, me ka antigen kikoʻī (inā e ʻimi ana i ka antibody i loko o ka serum) a i ʻole ka antibody kūikawā (inā e ʻimi ana i ka antigen i loko o ka serum).

C. Hemagglutination Inhibition Assays

Inā kiʻekiʻe ka manaʻo o ka maʻi maʻi i loko o kahi laʻana, ke hui ʻia ka hāpana me RBCs, e hana ʻia kahi lattice o nā maʻi a me nā RBC.Kapa ʻia kēia ʻano he hemagglutination.Inā loaʻa nā antibodies e kūʻē i nā hemagglutinins, e pale ʻia ka hematglutination.I ka wā o ka hoʻāʻo ʻana i ka hemagglutination inhibition, hui pū ʻia nā dilutions serial o ka serum me ka nui o ka maʻi maʻi.Ma hope o ka incubation, hoʻohui ʻia nā RBC, a waiho ʻia ka hui e noho no kekahi mau hola.Inā pale ʻia ka hemagglutination, hana ʻia kahi pellet o RBC ma lalo o ka paipu.Inā ʻaʻole pale ʻia ka hemagglutination, hoʻokumu ʻia kahi kiʻi ʻoniʻoni.