Mai Ianuali 2020 a ʻOkakopa 2020, ua mālama ʻia kahi noiʻi metodological i ka Halemai University Pisa i paʻi ʻia ma BMC Microbiology.Goldstream®Ua hoʻohana ʻia ʻo Fungus (1-3)-β-D-Glucan Test no ka ʻike ʻana i ka pae BDG mai nā laʻana BAL.Ua helu ʻia ka hopena e aMea Heluhelu Kinetic Tube IGL-200mai Era Biology.Hōʻike ka noiʻi e pono e noʻonoʻo ʻia ka BDG no kāna waiwai wānana maikaʻi ʻole.A he mea maikaʻi ia i ka hoʻoholo ʻana i ka maʻi o ka PCP no nā maʻi hoʻomalu maikaʻi ʻole.
Kāpae:
ʻO ke kūlana gula diagnostic o kēia manawa noPneumocystis jiroveciiua hōʻike ʻia e ka ʻike microscopic o ka haʻuki mai nā laʻana hanu hauʻoli, e like me ka wai bronchoalveolar-lavage, e wehewehe ana i ka "hōʻoia"P. jiroveciiʻo ka maʻi pneumonia, ʻoiai ua ʻae ʻo qPCR i ka wehewehe ʻana i ka maʻi "pilikia", no ka mea hiki ʻole ke hoʻokaʻawale i ka maʻi mai ka colonization.Eia nō naʻe, ʻoi aku ka wikiwiki o nā ʻano molekala, e like me ka end-point PCR a me qPCR, ʻoi aku ka maʻalahi o ka hana a me ka wehewehe ʻana, no laila e ʻae ai ka hale hana e hoʻihoʻi i ka ʻikepili microbiological pono i ke kauka i ka manawa pōkole.Ke manaʻo nei ka noiʻi ʻana i ka hoʻohālikelike ʻana i ka microscopy me nā molecular assays a me ka hana diagnostic beta-D-glucan ma nā wai bronchoalveolar-lavage mai nā maʻi me ka manaʻo kānalua.Pneumocystis jiroveciipneumonia.ʻO ka bronchoalveolar-lavage wai mai ʻumikūmāwalu kiʻekiʻe kiʻekiʻe a me ʻehā mau mea hoʻokele maikaʻi ʻole i hoʻopaʻa ʻia i ka methenamine kala kala a Grocott-Gomori, end-point PCR, RT-PCR, a me beta-D-glucan assay
Nā hualoaʻa:
ʻO nā laʻana bronchoalveolar-lavage microscopically maikaʻi a pau (50%) i hopena maikaʻi ʻia e ka helu hope a me ka PCR manawa maoli a me nā mea a pau, akā ʻelua, ua hopena maikaʻi ʻia ma ka helu beta-D-glucan.ʻIke ʻia ka PCR hope a me ka RT-PCR he 10 (55%) a me 11 (61%) mai loko mai o nā laʻana 18, no laila e hōʻike ana i ka hoʻonui ʻia ʻana i ka hoʻohālikelike ʻana i ka microscopy.ʻO nā RT-PCR āpau me kahi Ct<27 ua hōʻoia ʻia i ka microscopically, akā ʻaʻole nā mea hoʻohālike me kahi Ct≥ 27.
Nā hopena:
Hōʻike kā mākou hana i ka pono o ka hoʻoponopono hou ʻana a me ka wehewehe hou ʻana i ka hana o nā diagnostics molecular i kahi kūlana lapaʻau kūikawā, e like meP. jiroveciiʻO ka maʻi maʻi, he maʻi lapaʻau kakaʻikahi akā paʻakikī hoʻi a holomua wikiwiki e pili ana i nā pūʻali immunocompromised e pōmaikaʻi nui ʻia mai kahi maʻi wikiwiki.ʻO nā poʻe maʻi i koho ʻia, e like me nā pae hoʻohui, hiki ke hoʻokuʻu ʻia ka hopena maikaʻi ʻole e nā ʻano molecular.P. jiroveciipneumonia.
Franconi I, Leonildi A, Erra G, et al.Hoʻohālikelike i nā kaʻina hana microbiological like ʻole no ka ʻike ʻana i ka maʻi pneumonia Pneumocystis jirovecii ma ka wai bronchoalveolar-lavage.BMC Microbiol.2022;22(1):143.Hoʻopuka ʻia 2022 Mei 21. doi:10.1186/s12866-022-02559-1
Ka manawa hoʻouna: Iune-14-2022